Engineering bi-functional enzyme complex of formate dehydrogenase and leucine dehydrogenase by peptide linker mediated fusion for accelerating cofactor regeneration
نویسندگان
چکیده
منابع مشابه
Protein engineering of formate dehydrogenase.
NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs a...
متن کاملFast enzyme dynamics at the active site of formate dehydrogenase.
The role of femtosecond-picosecond structural dynamics of proteins in enzyme-catalyzed reactions is a hotly debated topic. We report infrared photon echo measurement of the formate dehydrogenase-NAD+-azide ternary complex. In contrast to earlier studies of protein dynamics, the data show complete spectral diffusion on the femtosecond-picosecond time scale with no static heterogeneity. This resu...
متن کاملEngineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae.
A eukaryotic formate dehydrogenase (EC 1.2.1.2, FDH) with its substrate specificity changed from NAD(+) to NADP(+) has been constructed by introducing two single-point mutations, Asp(196)-->Ala (D196A) and Tyr(197)-->Arg (Y197R). The mutagenesis was based on the results of homology modelling of a NAD(+)-specific FDH from Saccharomyces cerevisiae (SceFDH) using the Pseudomonas sp.101 FDH (PseFDH...
متن کاملSome properties of formate dehydrogenase.
In addition the enzyme catalyzes the oxidation of NADH by 0 2 . The stoichiometry of the first reaction is proved by the formation of the amount of NADH which under anaerobic conditions exactly corresponds to the amount of formate used. In this reaction NAD is replaceable by a large number of redox dyes. For the second reaction the stoichiometry is proved a) kinetically by the relation of the v...
متن کاملKinetics for Formate Dehydrogenase of Escherichia coli
Kinetic parameters of the selenium-containing, formate dehydrogenase component of the Escherichia coli formate-hydrogenlyase complex have been determined with purified enzyme. A ping-pong Bi Bi kinetic mechanism was observed. The K,,, for formate is 26 mM, and the K,,, for the electron-accepting dye, benzyl viologen, is in the range 1-5 mM. The maximal turnover rate for the formate-dependent ca...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2017
ISSN: 1618-0240
DOI: 10.1002/elsc.201600232